Fig. 1

Homozygous Zbtb11 inactivation in the fetal hematopoietic compartment drastically impairs FL mature lymphoid and myeloid output. (A) Schematic description of the analysis. Flow cytometry analysis of FL at E17.5: dot plots showing the representative incidence of (B/B′) pan leukocytes (CD45+); (C/C′) granulocytes (Gr1+), T cells (CD3+), and B cells (B220+). Scatter plots showing the absolute quantification of total content at E14.5, E16.5, and E17.5: (D) CD45+ leukocytes, (E) T cells, (F) B cells, and (G) granulocytes. Mouse embryo weight (H) and FL cellularity (I) showed no haploinsufficiency effect in heterozygotes. For panels D and G: E14.5 WT, n = 22 and KO, n = 7; E16.5 WT, n = 16 and KO, n = 4; E17.5 WT, n = 16 and KO, n = 5. For panels E and F: E14.5 WT, n = 22 and KO, n = 7; E16.5 WT, n = 9 and KO, n = 3; E17.5 WT, n = 16 and KO, n = 5. For panel H: E14.5 WT, n = 15, Het, n = 7, and KO n = 7; E16.5 WT, n = 10, Het, n = 12, and KO, n = 5; E17.5 WT, n = 12, Het, n = 4, and KO n = 4. For panel I: E14.5 WT, n = 15, Het, n = 7, and KO, n = 7; E16.5 WT, n = 8, Het, n = 8, and KO, n = 4; E17.5 WT, n = 12, Het, n = 4, and KO n = 5. WT/controls; (green); KO, Zbtb11hKO (red); Het, Zbtb11WT/hKO (blue); data ± standard error of the mean. Two-way analysis of variance with Tukey (black) or Šídák (blue) multiple comparisons: ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001.