Figure 5

Ramipril and MDIVI-1 treatments lower viral burden in IAV-infected zebrafish. (A) Viral titers for both ramipril (0.2 nM)- and MDIVI-1 (7 nM)-treated 2 dpf zebrafish infected with PR8 were significantly higher at 24 and 48 hpi when compared to 0 hpi, but not different at 72 hpi. For the DMSO-treated zebrafish, viral titers were significantly increased at 24, 48, and 72 hpi (p < 0.0001 for each comparison). For the ramipril-treated zebrafish, viral titers were significantly increased at 24 (p < 0.0001) and 48 hpi (p = 0.0022). For the MDIVI-1-treated zebrafish, viral titers were also significantly increased at 24 and 48 hpi (p < 0.0001 for each comparison). (B) Viral titers for ramipril- and MDIVI-1-treated 3 dpf zebrafish infected with PR8 were significantly lower by 48 hpi (p < 0.0001 for each comparison). (C) Viral titers for ramipril- and MDIVI-1-treated 3 dpf zebrafish infected with mVenus-PR8 were significantly lower at 48 hpi (p < 0.0001 for each comparison). TCID₅₀ assays were conducted using n = 3 and 25 larvae per group for each time point. Not significant (ns), p > 0.05; ** p < 0.01; **** p < 0.0001.