Figure 1

Characterization of PR8 and Color-flu systemically infected AB zebrafish. (A) Decreased survival of AB zebrafish systemically infected with three different lots of PR8 IAV at 2 dpf compared to vehicle (HBSS) controls (p < 0.0001 for each lot comparison). Survival rates of PR8-infected embryos were not significantly different between lots (p = 0.0603). (B) Decreased survival of AB zebrafish systemically infected with two different lots of PR8 IAV at 3 dpf compared to vehicle controls (p < 0.0001 for each lot comparison). Survival rates of PR8-infected embryos were not significantly different between lots (p = 0.1442). (C) Decreased survival of AB zebrafish systemically infected with eCFP-PR8 or Venus-PR8 (3.2 × 102 TCID₅₀/mL) compared to vehicle controls (p < 0.0001 for each comparison). Survival rates of eCPF-PR8- or Venus-PR8-infected zebrafish were not significantly different strains (p = 0.5238). (D) Increased TCID₅₀ viral titer in Color-flu systemically infected AB zebrafish at 24 and 48 hpi compared to 0 hpi. eCFP-PR8-, mCherry-PR8-, and Venus-PR8-infected zebrafish had increased viral titers at 24 and 48 hpi compared to 0 hpi (adjusted p-values = 0.0001 and <0.0001, respectively, for eCFP-PR8; 0.0019 and 0.0001, respectively, for eGFP-PR8; 0.0047 and <0.0001, respectively, for mCherry-PR8; and 0.0003 and <0.0001, respectively, for Venus-PR8). Survival studies were conducted with four independent experiments (n = 4) and 50 larvae per sample group. TCID₅₀ assays were carried out with three independent experiments (n = 3) and 25 larvae per group for each time point. Not significant (ns), p > 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.