FIGURE

Figure 5

ID

ZDB-FIG-240129-189

Publication

Sun et al., 2024 - amer1 Regulates Zebrafish Craniofacial Development by Interacting with the Wnt/β-Catenin Pathway

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Figure 5

Genotypes and phenotypes of F2 zebrafish. (A) Sequencing results and brightfield images of F2 zebrafishes at 3 and 4 dpf with five different genotypes/treatments: wildtype, amer1^+/−, amer1^−/−, and amer1^−/− + upf3a MO, wildtype + upf3a MO. amer1^−/− F2 embryos treated with upf3a MO showed malformation of the mandible. Red suqares indicate mutated sites. NA: not applicable. (B) Fluorescence staining showing that chondrocytes were regularly distributed in wildtype and amer1^−/− F2 embryos, but were disarranged in amer1^−/− F2 embryos treated with upf3a MO.

Expression Data

Gene:	EGFP
Fish:	ba2Tg (TU) ba2Tg + CRISPR1-amer1 (TU) amer1^{zf4147/zf4147}; ba2Tg + MO1-upf3a (TU)
Knockdown Reagents:	CRISPR1-amer1 MO1-upf3a
Anatomical Term:	chondrocyte
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	amer1^{zf4147/zf4147} (TU) TU + MO1-upf3a amer1^zf4147/+ (TU) amer1^{zf4147/zf4147}; ba2Tg (TU) amer1^{zf4147/zf4147} + MO1-upf3a (TU) amer1^{zf4147/zf4147}; ba2Tg + MO1-upf3a (TU)
Knockdown Reagent:	MO1-upf3a
Observed In:	cranial cartilage chondrocyte ventral mandibular arch
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.