Phenotypic rescue of F0 amer1 knockdown embryos using IWR-1-endo. (A) Schematic diagram of the rescue and observation process. Different numbers indicate different treatment. (B) Proportions of malformed wildtype, amer1 knockdown and IWR-treated amer1 knockdown embryos, showing that the proportion of malformed IWR-treated amer1 knockdown was significantly lower than that of amer1 knockdown embryos. (C) Representative brightfield images of wildtype, amer1 knockdown embryos, and IWR-treated amer1 knockdown embryos. (D) Representative Alcian staining images of wildtype (first row, left), IWR-treated wildtype (first row, middle), amer1 knockdown (first row, right), and IWR-treated amer1 knockdown embryos showing normal development of pharyngeal arch cartilage (second row, left), partial loss (second row, middle), and complete loss (second row, right). ** p < 0.01; *** p < 0.001.
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