Fig 6

Kidney marrow blasts expressing rag2:EGFP-CA-CaMKII are B lineage and have reduced productive IgH VDJ rearrangements.

EGFP positive and negative lymphoid cells from the kidney marrow were sorted from six-nine month old adult rag2:EGFP-CA-CaMKII transgenics on a wild-type or tp53 mutant background and the expression of B (rag2, igiv1s1, cd79a, ighm-C, igt-C, pax5, ikzf1) and T (lck, tcrd-C) lineage markers was determined by RT-PCR. Ef1a served as control (A). Arrangement of the zebrafish IgH locus and schematic of IgHM and IgHT VDJ assembly (B). Genomic analysis of VDJ recombined (red arrow) IgVH1-Jm, IgVH2-Jm, IgVH3-Jm, IgVH1-Jt, IgVH2-Jt, and IgVH3-Jt in rag2:DsRed, rag2:EGFP-CA-CaMKII, tp53 mutant, and rag2:EGFP-CA-CaMKII; tp53 mutant kidney marrow lymphocytes using semi-nested PCR. Ef1a served as a control (C). Analysis of productive IgHM (D) and IgHT (E) rearrangements in genomic DNA from rag2:EGFP-CA-CaMKII, tp53 mutant, and rag2:EGFP-CA-CaMKII; tp53 mutant kidney marrow lymphocytes. P values were calculated using Fisher’s exact test. * p<0.05, ** p<0.01.