Fig. 2

Proliferation and 2D-migration of MDA-MB-231 cells are independent of ANXA2. A, B Proliferation of control or shANXA2 MDA-MB-231 cells was monitored with a lensfree microscope enabling long-term observation of a large cell population [45]. A Averaged number of cells (± SEM) normalized to the number of seeded cells from three independent experiments. B Normalized total cell biomass (± SEM), which considers individual number and cell size, from three independent experiments. C, D In vitro 2D-migration assay was performed on control or shANXA2 MDA-MB-231 cells. Cells were plated into culture-Insert 2-well in 35 mm µ-Dish (Ibidi) coated with 0.5 mg/mL gelatin. Cell progression was monitored with a lensfree microscope, after removing the culture insert. C Typical images of control or shANXA2 MDA-MB-231 cells immediately or 7h after insert release are presented. Migration front is drawn in yellow. D Mean gap closure kinetics and cell velocity measure (± SEM) from three independent experiments are presented for each cell type. Nonparametric Mann–Whitney test. ***p < 0.001