Figure 6

Schematic summary. Tg(ifih1_mut) crossed with the inflammation and type I interferon reporters, nfkb:eGFP and isg15:eGFP, activate the expression of GFP 3 days after the injection of poly I:C. Larvae bearing the mutation p.Arg742His show the red eye tag, and larvae bearing the isg15:eGFP reporter show the green heart tag marker. The genetic inhibition of mavs or ikbke prevents the transduction of the signal from Mda5 after sensing the poly I:C, avoiding the activation and translocation of Irf3/irf7 and Nfkb into the nucleus and preventing the expression of type I interferon and proinflammatory cytokines and the GFP activation. The chemical inhibition by the addition of Baricitinib by bath immersion provokes the blockade of the signal transduction through the Jak1 and Jak2 kinases, which results in the prevention of Stat and Irf9 translocation into the nucleus and activation of the ISGs and proinflammatory cytokine expression, avoiding the activation of GFP of the reporter lines.