Figure 5

Pharmacological inhibition of Jak impairs the induction of ISGs and inflammation in Tg(ifih1_mut) upon poly I:C stimulation. (A)nfkb:eGFP and isg15:eGFP zebrafish were crossed with Tg(ifih1_mut), eggs were injected with poly I:C at 0 dpf, larvae were dechorionated at 1 dpf, and baricitinib was added to the water and renewed at 2 dpf, and images were taken and analyzed by microscopy at 3 dpf. (B–E) Nfkb activity (B, C) and isg15 levels (D, E) were analyzed by fluorescence microscopy and quantified. Each dot represents a larva and the mean ± SEM for each experimental group is also shown. Representative merge images of whole larvae were also shown (B, D). (F, G) Transcript levels of isg15(F) and stat1(G) in larvae from the cross of nfkb:eGFP and Tg(ifih1_mut) were analyzed by RT-qPCR and the results are shown as the mean ± SEM of pooled larvae. p-values were calculated using one-way ANOVA and Tukey multiple range test. ns, not significant; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.