FIGURE

Figure 4

ID

ZDB-FIG-231212-31

Publication

Bernal-Bermúdez et al., 2023 - A zebrafish model of Ifih1-driven Aicardi-Goutières syndrome reproduces the interferon signature and the exacerbated inflammation of patients

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Figure 4

Genetic inhibition of Mavs/Ikbke signaling pathway impairs the induction of inflammation and ISGs in Tg(ifih1_mut) upon poly I:C stimulation. Tg(ifih1_mut) were crossed with nfkb:eGFP and isg15:eGFP reporter lines, injected with poly I:C and crRNA/Cas9 complexes for mavs or ikbke. Nfkb activity (A, B) and isg15 levels (C, D) were analyzed by fluorescence microscopy and quantified. Each dot represents a larva and the mean ± SEM for each experimental group is also shown. Representative merge images of whole larvae were also shown (A, C). (E) Transcript levels of isg15 in larvae from the cross of nfkb:eGFP and Tg(ifih1_mut) were analyzed by RT-qPCR and the results are shown as the mean ± SEM of pooled larvae. p-values were calculated using one-way ANOVA and Tukey multiple range test. **p ≤ 0.01, ****p ≤ 0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Immunol