FIGURE

Figure 2

ID

ZDB-FIG-231127-89

Publication

Deng et al., 2023 - Dynamic Regulation of brsk2 in the Social and Motor Development of Zebrafish: A Developmental Behavior Analysis

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Figure 2

Generation of brsk2ab mutant zebrafish. (A) structure modelling of zebrafish brsk2a and brsk2b protein. PK represents protein kinase domain; UBA represents ubiquitin-associated domain. (B) Two CRISPR/Cas9 guide RNAs were designed to target exon 10 in brsk2a and exon 4 in brsk2b, respectively. The mutations of brsk2a and brsk2b were verified by Sanger sequencing, and computational modelling showed the proteins were truncated through SWISS-MODEL tool (https://swissmodel.expasy.org/interactive, accessed on 12 July 2023). (C) To validate the knockout efficiency, total mRNA was extracted at 3 mpf followed by reverse transcription, and qRT-PCR was conducted to validate the expression level of brsk2a, and brsk2b was decreased in brsk2ab^−/− zebrafish (n = 3 for each genotype, brsk2a, p = 0.0007; brsk2b, p = 0.0004). Data are shown as mean ± SEM, *** p < 0.001.

Expression Data

Genes:	brsk2a brsk2b
Fish:	TU brsk2a^{zf3919/zf3919}; brsk2b^{zf3706/zf3706} (TU)
Anatomical Term:	brain
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	brsk2a^{zf3919/zf3919}; brsk2b^{zf3706/zf3706} (TU)
Observed In:	brain
Stage:	Adult

Phenotype Detail

Acknowledgments

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