Fig. 2.

Delaminated otic NBs dynamically change their shape, producing filopodia and membrane protrusions. (A) Single-cell labelling of delaminating NBs with CRISPR Eraser in Tg(neurod:eGFP). Delaminating NBs undergo apical thinning and delamination (white arrowheads in top row) right after starting to express neurod (asterisks). Subsequently, they acquire a much more rounded, mesenchymal shape and produce membrane protrusions (white arrowheads, middle row). Finally, membrane protrusions precede large membrane deformations (white brackets, bottom row). Representative of n=7. (B) Example of the change in roundness of one NB through the different phases. Phases are colour-coded and a 3D IMARIS reconstruction is shown on the right. (C) NBs produce dynamic filopodia after delamination (coloured arrowheads). Bottom row shows how a membrane protrusion becomes a much bigger membrane deformation (white arrow). Panels on the right show cells in left-hand panels depicted inside the dashed lines with eGFP staining from Neurod⁺ cells shown in magenta. Scale bars: 10 µm.