PUBLICATION
Pioneer statoacoustic neurons guide neuroblast behaviour during otic ganglion assembly
- Authors
- Bañón, A., Alsina, B.
- ID
- ZDB-PUB-231109-4
- Date
- 2023
- Source
- Development (Cambridge, England) 150(21): (Journal)
- Registered Authors
- Alsina, Berta
- Keywords
- CRISPR, Delamination, Inner ear, Migration, Neuroblasts, RhoGTPases, Statoacoustic ganglion, Zebrafish
- MeSH Terms
-
- Animals
- Cell Differentiation/genetics
- Ear, Inner*/metabolism
- Hair Cells, Auditory/physiology
- Sensory Receptor Cells
- Zebrafish*/genetics
- PubMed
- 37938828 Full text @ Development
Citation
Bañón, A., Alsina, B. (2023) Pioneer statoacoustic neurons guide neuroblast behaviour during otic ganglion assembly. Development (Cambridge, England). 150(21):.
Abstract
Cranial ganglia are aggregates of sensory neurons that mediate distinct types of sensation. The statoacoustic ganglion (SAG) develops into several lobes that are spatially arranged to connect appropriately with hair cells of the inner ear. To investigate the cellular behaviours involved in the 3D organization of the SAG, we use high-resolution confocal imaging of single-cell, labelled zebrafish neuroblasts (NBs), photoconversion, photoablation, and genetic perturbations. We show that otic NBs delaminate out of the otic epithelium in an epithelial-mesenchymal transition-like manner, rearranging apical polarity and primary cilia proteins. We also show that, once delaminated, NBs require RhoGTPases in order to perform active migration. Furthermore, tracking of recently delaminated NBs revealed their directed migration and coalescence around a small population of pioneer SAG neurons. These pioneer SAG neurons, not from otic placode origin, populate the coalescence region before otic neurogenesis begins and their ablation disrupts delaminated NB migratory pathways, consequentially affecting SAG shape. Altogether, this work shows for the first time the role of pioneer SAG neurons in orchestrating SAG development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping