FIGURE

Figure 1—figure supplement 1.

ID

ZDB-FIG-231013-21

Publication

Phelps et al., 2023 - Hybridization led to a rewired pluripotency network in the allotetraploid Xenopus laevis

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Figure 1—figure supplement 1.

Measuring genome activation.

(A) (Top) Animal and vegetal views of embryos treated with DMSO (vehicle) versus triptolide. Triptolide-treated embryos fail to gastrulate. (Bottom) Comparison of DMSO versus cycloheximide treated embryo. Treatment was at stage 8, which inhibits progression to stage 9. Scale bar = 0.5 mm. (B) Biplot of RNA-seq for untreated versus DMSO-treated embryos at stage 9, showing no effect on the transcriptome. (C) Biplot of RNA-seq for DMSO versus triptolide treated embryos, showing inhibited activation as detected by exonic (purple) and intronic (green) signal. The predicted mir-427 primary transcript is labeled and exhibits >95% expression inhibition. (D) Histogram of mRNA percent identity between homeolog pairs, as measured by Needleman-Wunsch alignment. Maximum is 0.975 (5 in every 200 bases, which should be generally distinguishable by RNA-seq using 2x100 sequencing reads).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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