Fig. 5

Mineralization status of zebrafish juveniles fed diets supplemented with the ethanolic extracts of Skeletonema costatum (SKLT) and Tetraselmis striata CTP4 (CTP4). A Heatmap displaying the group modal values for the mineralization index assigned to each skeletal structure. B Schematic representation of the modal mineralization status of the fish fed CTRL, SKLT 2.5% or CTP4 2.5% (from top to bottom). C Representative images of fish illustrated in C. D Content of phosphorus (P) and calcium (Ca), and Ca/P ratio in juveniles fed experimental diets. E Expression of marker genes for osteoblastic differentiation, ECM mineralization, and osteoclast function. For gene expression, differences were tested through Student’s t test. Asterisks indicate p < 0.0332 (*), p < 0.0021. (**), p < 0.0002 (***) and p < 0.0001 (****). J jaws, Op + Ba operculum and branchial arches, Vert vertebral bodies, NA + S neural arches and spines, HA + S haemal arches and spines, Uro caudal vertebrae bodies and urostyle