Assessment of reproductive success in WT (white bars and data points) and avp-/- (purple bars and data points) zebrafish. (A) Percent successful breeding trials in WT and avp-/- breeding pairs analyzed by Fisher’s exact test. (B) (Median) number of fertilized eggs in WT and avp-/- in all breeding trials analyzed by Mann-Whitney U test. Asterisk indicates a significant difference at P<0.01. (C) (Mean) clutch size expressed as the number of fertilized eggs in successful breeding trials analyzed by t-test. Asterisk indicates a significant difference at P<0.001. (D) Full-factorial crossbreeding experiments to delineate sex-specific contributions of the avp genoptype to reproductive success. Four crosses were bred: WT females x WT males; WT females x avp -/- males; WT males x avp -/- females; avp-/- males x avp-/- females. (D) (Median) number of fertilized eggs of each breeding trial across breeding groups analyzed by two-way ANOVA on ranks followed by Scheirer-Ray-Hare extension. (E) (Mean) Clutch size expressed as the number of fertilized eggs in successful breeding trials. Data were analyzed by two-way ANOVA (F).
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