(A) Larval cortisol concentration at 5 dpf and (B) baseline cortisol concentration in sexually mature adult blood samples. Larval cortisol was determined in WT and avp-/- 5 dpf larvae pools (n=4 per genotype, 20 larvae per pool) according to published protocols (50). Adult whole blood cortisol was analyzed in adults of mixed sex and analyzed under baseline conditions for both WT (n=10) and avp-/- mutants (n=10) according to published protocols (51) with the modification that cortisol was extracted from whole blood. Data are presented as means ± S.E.M. and were analyzed using a t-test. A threshold of P<0.05 was used to discern a significant difference, which is indicated by asterisk.
|