Fig. 5.

Exposure to Shigella induces epigenetic reprogramming of neutrophils.

(A) Isolation of neutrophils by FACS from naïve and Shigella-trained larvae for ChIP-seq on H3K4me3 modification. (B) Venn diagram showing the number of common and unique genes marked by H3K4me3 peaks in their promoter regions [±3 kb from transcription start site (TSS)] of PBS and Shigella ∆T3SS–trained larvae. (C) Enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways (P value < 0.01) associated with the 2052 unique Shigella ∆T3SS training genes marked by an H3K4me3 peak in their promoter regions (±3 kb from TSS). FoxO, Forkhead box O; ErbB, erythroblastic oncogene B; VEGF, vascular endothelial growth factor; TGF-β, transforming growth factor–β. (D) Venn diagram showing the number of common and unique genes marked by H3K4me3 peaks in their promoter regions (±3 kb from TSS) of PBS and Shigella WT trained larvae. (E) Enriched KEGG pathways (P value < 0.01) associated with the 1612 unique Shigella WT training genes marked by an H3K4me3 peak in their promoter regions (±3 kb from TSS). NOD, nucleotide binding oligomerization domain.