FIGURE

Fig. 2

ID

ZDB-FIG-230810-5

Publication

Yao et al., 2023 - DExH/D RNA helicase 15 regulates zebrafish intestinal development through the Wnt signaling pathway

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Fig. 2

Fig. 2. Dhx15 is essential for zebrafish intestinal development. (A-E A'-E') WISH analysis of fabp2 (A A'), apoa1a (B B′), apoa4b.1 (C C′), aqp3a (D D′), fabp6 (E E') expression in zebrafish embryos at 3.5 dpf. The expression of fabp2 (expressed in small intestinal epithelial cells), apoa1a, apoa4b.1 (expressed in the gut), aqp3a (expressed in the gastrointestinal tract), and fabp6 (expressed in midgut) were dramatically deceased in dhx15 knockout compared with the wide-type zebrafish. (F-J F′-J') WISH analysis of fabp2 (F F′), apoa1a (G G'), apoa4b.1 (H H′), aqp3a (I I′), fabp6 (J J') expression in zebrafish embryos at 60 hpf. The expression of fabp2, apoa4a, apoa4b.1, aqp3a, and fabp6 were marginal decreased in dhx15 knockout compared with the wide-type zebrafish. (K-O K′-O′) WISH analysis of fabp2, apoa4a, apoa4b.1, aqp3a, fabp6 expression in zebrafish at 3.5 dpf. The expression of fabp2, apoa4a, apoa4b.1, aqp3a, and fabp6 in dhx15^−/−;Tg (hsp70:DHX15-2A-mcherry) zebrafish were restored (K′-O′) after overexpressing DHX15 by conditional heat activation. The arrows indicate the intestine of zebrafish.

Expression Data

Genes:	apoa1a apoa4b.1 aqp3a fabp2 fabp6
Fish:	AB dhx15^{zf3775/zf3775} (AB) dhx15^{zf3775/zf3775}; zf3777Tg (AB)
Condition:	heat shock
Anatomical Terms:	gut intestinal epithelium mid intestine
Stage Range:	Pec-fin to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dhx15^{zf3775/zf3775} (AB) dhx15^{zf3775/zf3775}; zf3777Tg (AB)
Condition:	heat shock
Observed In:	gut intestinal epithelium mid intestine
Stage Range:	Pec-fin to Protruding-mouth

Phenotype Detail

Acknowledgments

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