FIGURE

Fig. 3

ID
ZDB-FIG-230810-6
Publication
Yao et al., 2023 - DExH/D RNA helicase 15 regulates zebrafish intestinal development through the Wnt signaling pathway
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Fig. 3

Fig. 3. Increased apoptosis and decreased proliferation were observed in dhx15 knockout zebrafish. (A) Representative images of PH3 immunofluorescence staining in dhx15+/+ and dhx15−/− zebrafish at 3.5 dpf. (B) The number of PH3-positive cells in the intestine of dhx15+/+ was significantly higher than that of dhx15−/− embryos at 3.5 dpf (P < 0.0001 by student t-test). (C) Representative images of Caspase3 immunofluorescence staining in dhx15+/+ and dhx15−/− zebrafish at 3.5 dpf. (D) The number of Caspase3 positive cells in the intestine of dhx15+/+ was significantly lower than that of dhx15−/− embryos at 3.5 dpf (P < 0.0001 by student t-test). (E) Representative images of PH3 immunofluorescence staining in dhx15+/+ and dhx15−/− zebrafish at 60 hpf. (F) The number of PH3-positive cells in the intestine of dhx15+/+ was comparable to that of dhx15−/− embryos at 60hpf (by student t-test). (C) Representative images of Caspase3 immunofluorescence staining in dhx15+/+ and dhx15−/− zebrafish at 60 hpf. (D) The number of Caspase3 positive cells in the intestine of dhx15+/+ was comparable to that of dhx15−/− embryos at 60 hpf (by student t-test). The red box outlined the location of immunofluorescence staining in the embryos. ****P < 0.0001, NS: not significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage: Protruding-mouth

Phenotype Detail
Acknowledgments
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