FIGURE
Fig. 7
Fig. 7
Effect on AmyM secretion. B. subtilis BWB09 (ΔxynA ΔamyE) carrying pCS73 (PamyQ–amyM) to express amyM from the constitutive PamyQ promoter was grown until mid-log phase and treated with different concentrations of ES24 or NFT for 3 h prior to SDS-PAGE and Western blotting. (A) Western blot analysis of culture supernatant using an α-AmyM antibody (top) and of cell lysate using an α-streptavidin antibody detecting the cytosolic protein PycA (bottom). (B) Quantification of pixel intensity in AmyM bands. **p < 0.01. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
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