FIGURE

Fig. 2

ID

ZDB-FIG-230718-33

Publication

Schäfer et al., 2023 - Dual Action of Eeyarestatin 24 on Sec-Dependent Protein Secretion and Bacterial DNA

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Fig. 2

Oxidative stress assays. (A) Oxyburst Green assay for detecting ROS in B. subtilis 168CA. The fluorescent probe shows a marked increase in fluorescence when ROS are present. It should be noted that Oxyburst Green has been reported to be specific for the detection of superoxide, yet we have found that it reacts to different sources of ROS (see Figure S2). (B) Influence of ROS scavengers on antibiotic activity against B. subtilis 168CA. MICs were determined in the absence or presence of the superoxide scavenger tiron or the hydroxyl radical scavenger thiourea. If one of these ROS species contributes to the activity of the compound, the MIC should increase in the presence of the respective scavenger.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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