FIGURE

Figure 1

ID

ZDB-FIG-230707-90

Publication

Yue et al., 2023 - VBP1 negatively regulates CHIP and selectively inhibits the activity of hypoxia-inducible factor (HIF)-1α but not HIF-2α

Other Figures

All Figure Page

Back to All Figure Page

Figure 1

Generation of the transgenic HRE-sv40mp:GFP reporter zebrafish.A, Schematic representation of the Tol-2 vector used to generate the Tg (hre-sv40mp: GFP) zebrafish. The construct includes a 68-bp fragment from the hypoxia-response element (HRE) of the human ENO1 gene and the SV40 minimal promoter (SV40mp). B, Representative images show the Tg (hre-sv40mp:GFP) embryos at 48 and 72 hpf under normoxic or hypoxic conditions. Tg (hre-sv40mp:GFP) embryos were exposed to hypoxia (10% O₂) for 24 or 48 h from 24 hpf. Transgenic embryos show strong GFP expression during hypoxia. However, GFP expression is observed only in the visceral organs in the transgenic embryos exposed to normoxia. C, Tg(hre-sv40mp:GFP) embryos injected with the dominant-active (DA) HIF-1α mRNA at the 1-cell stage show strong GFP expression at 48 hpf. D, Transgenic zebrafish in the vhl^−/− background show strong GFP fluorescence compared to the sibling embryos at 72 hpf. Scale bar, 500 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Biol. Chem.