FIGURE

Fig. 5

ID

ZDB-FIG-230707-46

Publication

Dinarello et al., 2023 - STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia

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Fig. 5

stat3 genetic ablation affects angiogenesis, and macrophage migration.

A, B Representative pictures and fluorescence quantification of the trunk of 54-hpf stat3^+/+, stat3^+/− and stat3^−/− in Tg(Fli1:EGFP)^y1 transgenic background incubated in normoxia and hypoxia for 6 h. Scale bar: 1 mm. n = 15 stat3^+/+ normoxia; 17 stat3^+/+ hypoxia; 21 stat3^+/− normoxia; 20 stat3^+/− hypoxia; 14 stat3^−/− normoxia; 14 stat3^−/− hypoxia (larvae used for this experiment came from three independent breeding between stat3^+/− zebrafish). C, D Representative pictures and fluorescence quantification of the tail of 54-hpf stat3^+/+, stat3^+/− and stat3^−/− larvae in Tg(gata1:dsRed)^sd2 transgenic background incubated in normoxia and hypoxia. Scale bar: 1 mm. n = 18 stat3^+/+ normoxia; 14 stat3^+/+ hypoxia; 41 stat3^+/− normoxia; 44 stat3^+/− hypoxia; 17 stat3^−/− normoxia; 20 stat3^−/− hypoxia (larvae used for this experiment came from four independent breeding between stat3^+/− zebrafish). E, F Representative pictures of 54-hpf control (ctrl inj) and hif1α morphants (MO-hif1α) in Tg(LysC:dsRed)^nz50 transgenic background incubated in normoxia and hypoxia for 6 h (scale bar: 500 μm) quantification of the ratio of dsRed-positive cells in region A and in region B. Area B between dashed lines. Arrowheads point at fluorescent cells in Area A. n = 8 ctrl inj normoxia; 9 ctrl inj hypoxia; 7 MO-hif1α normoxia; 8 MO-hif1α hypoxia (larvae used for this experiment came from three independent breeding between wild type zebrafish) G, H Representative pictures of 54-hpf stat3^+/+, stat3^+/− and stat3^−/− larvae in Tg(LysC:dsRed)^nz50 transgenic background incubated in normoxia and hypoxia for 6 h (scale bar: 500 μm); quantification of the ratio of dsRed-positive cells in region A and in region B. Area B between dashed lines. Arrowheads point at fluorescent cells in Area A. n = 11 stat3^+/+ normoxia; 16 stat3^+/+ hypoxia; 22 stat3^+/− normoxia; 23 stat3^+/− hypoxia; 11 stat3^−/− normoxia; 17 stat3^−/− hypoxia (larvae used for this experiment came from three independent breeding between stat3^+/− zebrafish). Mean ± SEM.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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