FIGURE

Fig. 4

ID

ZDB-FIG-230707-45

Publication

Dinarello et al., 2023 - STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia

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Fig. 4

Stat3 and Hif1α cooperate in the nucleus for the transcription of Hif1α target genes.

A, B Representative pictures and fluorescence quantification of 4-dpf HRE:mCherry reporter larvae treated with either DMSO or 50 μM AG490 for 24 h after injection of hif1ab DA mRNA. n = 3 independent biological replicates; 10 DMSO, 20 DMSO hif1ab DA mRNA, 19 AG490, 24 AG490 hif1ab DA mRNA (larvae used for this experiment came from three independent breeding between wild type zebrafish). Scale bar 500 μm. C RT-qPCR analysis of vegfa of 4-dpf larvae injected with hif1ab DA mRNA and treated with AG490 (n = 4 independent biological replicates composed by pool of 15 larvae, each dot represents a pool of larvae). D RT-qPCR analysis of hk1 of 4-dpf larvae injected with hif1αb DA mRNA and treated with AG490 (n = 4 independent biological replicates composed by pool of 15 larvae, each dot represents a pool of larvae). E RT-qPCR analysis of vegfa, hk1, egln3, and hif1αb on EGFP-positive and EGFP-negative cells sorted from SBE:EGFP transgenic adult intestines (three adult intestines were pooled together in two biological replicas). Mean ± SEM.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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