FIGURE

Figure 6

ID

ZDB-FIG-230703-12

Publication

Henderson et al., 2023 - Proteinase 3 promotes formation of multinucleated giant cells and granuloma-like structures in patients with granulomatosis with polyangiitis

Other Figures

All Figure Page

Back to All Figure Page

Figure 6

MGC formation is dependent on MAC-1, PAR-2 and IL-6. The effect of MAC-1 and IL-6 inhibition was tested in patients with GPA by culturing monocytes with enzymatically active or inactive PR3 and measuring MGC formation in the presence or absence of anti-MAC-1 antibody, anti-IL-6 antibody or control immunoglobulin. Consistently, PR3 stimulated an increase in MGC formation as well as in the presence of control immunoglobulin with enzymatically active and inactive PR3 (A, B). Anti-MAC-1 antibody and anti-IL-6 antibody reduced MGC formation in both conditions, and the effect of combined anti-MAC-1 antibody and anti-IL-6 antibody also significantly reduced MGC formation. Values plotted as median and 95% CI; *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. Difference between conditions determined by one-way ANOVA tests. ANOVA, analysis of variance; IL, interleukin; MGC, multinucleated giant cells; PAR-2, protease-activated receptor-2; PR3, proteinase 3.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Ann. Rheum. Dis.