ZFIN Image: Henderson et al., 2023, Figure 6

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Figure 6

MGC formation is dependent on MAC-1, PAR-2 and IL-6. The effect of MAC-1 and IL-6 inhibition was tested in patients with GPA by culturing monocytes with enzymatically active or inactive PR3 and measuring MGC formation in the presence or absence of anti-MAC-1 antibody, anti-IL-6 antibody or control immunoglobulin. Consistently, PR3 stimulated an increase in MGC formation as well as in the presence of control immunoglobulin with enzymatically active and inactive PR3 (A, B). Anti-MAC-1 antibody and anti-IL-6 antibody reduced MGC formation in both conditions, and the effect of combined anti-MAC-1 antibody and anti-IL-6 antibody also significantly reduced MGC formation. Values plotted as median and 95% CI; *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. Difference between conditions determined by one-way ANOVA tests. ANOVA, analysis of variance; IL, interleukin; MGC, multinucleated giant cells; PAR-2, protease-activated receptor-2; PR3, proteinase 3.

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