Fig. 6

Analysis of mRNA expression levels of UBAP2 and osteoblast and osteoclast differentiation-related genes in the peripheral blood samples from normal controls and postmenopausal women with osteoporosis.

Relative mRNA expression and fold changes in expression of aUBAP2, osteoblast differentiation markers bALPL and cBGLAP, and osteoblast differentiation markers dTNF, eACP5, and fCTSK between whole blood-derived buffy coats from normal controls and patients with osteoporosis are shown. Total RNAs were isolated from buffy coat of peripheral blood samples from normal control postmenopausal women (n = 32) and postmenopausal women with osteoporosis (n = 31) and then used as templates to synthesize cDNAs. Relative mRNA expression of the six genes relative to the internal control GUSB was determined by quantitative reveres-transcription PCR. The negative values of delta cycle threshold (–ΔCt) for UBAP2 are plotted by opened and closed circles. The minimum to maximum values and median values are shown in box-and-whisker plots. All experiments were repeated three times with duplicated samples. Exact p-values between the groups were calculated with an unpaired two-tailed Student’s t-test. Source data are provided as a Source Data file.