Fig. 4

Analysis of mRNA expression levels of UBAP2 and osteoblast and osteoclast differentiation-related genes in the bone marrow samples from postmenopausal woman controls and postmenopausal woman patients with osteoporosis.

Relative mRNA expression and fold changes in the expression of aUBAP2, osteoblast differentiation markers bALPL, cBGLAP, dTNF, eACP5, and fCTSK in bone marrow-derived buffy coats from controls and patients with osteoporosis are shown. Total RNAs were isolated from buffy coat of bone marrow samples from the normal postmenopausal woman controls (n = 15) and postmenopausal women with osteoporosis (n = 30) and then used as templates to synthesize cDNAs. Relative mRNA expression of the six genes relative to the internal control GUSB was determined via quantitative reverse-transcription PCR. The negative values of the delta cycle threshold (–ΔCt) for UBAP2 are plotted via open and closed circles. The minimum to maximum values and median values are shown in box-and-whisker plots. All experiments were repeated three times with duplicated samples. Exact p-values between the groups were calculated with an unpaired two-tailed Student’s t-test. Source data are provided as a Source Data file.