FIGURE

Fig. 6

ID

ZDB-FIG-230616-11

Publication

Tabler et al., 2022 - Loss of glyoxalase 2 alters the glucose metabolism in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

glo2^−/−zebrafish showed a reduction of liver P70–S6 kinase activation while maintaining euglycemia and unchanged AKT phosphorylation.A) The quantification of the pAKT Western blot in glo2^−/− livers showed no change in phosphorylation in the postprandial liver compared to the liver of wildtype zebrafish, indicating intact insulin signaling in glo2^−/− livers. B) The total amount of AKT was unchanged in glo2^−/− and glo2^+/+ zebrafish liver. C) The quantification of the housekeeping gene β-actin showed no significant difference in signal volume, indicating equal loading in all samples. D) The expression of insulin receptor A/B mRNA remained unaltered in glo2^−/− zebrafish. Measured using RT-qPCR, normalized to b2m. N = 4. E)glo2^−/− and glo2^+/+ zebrafish showed similar blood glucose kinetics from fasting to 3 h postprandial state. N = 3-5 adult zebrafish. F)glo2^−/− zebrafish showed a reduction of the phosphorylation of P70–S6 kinase. N = 3/4. G) The relative amount of myo-inositol was increased in glo2^−/− zebrafish. Determined through GC/MS. N = 4. One datapoint represents one zebrafish. N = 3/4. For statistical analysis normality tests were used (A, B, C, D, E, F, G), followed by unpaired t-tests (A, B, C, D, E, F, G). Data presented as mean ± SD. ns = p > 0.05, * = p < 0.05. insr: insulin receptor, pAKT: phosphorylated AKT, tAKT: total AKT, GC-MS: gas chromatography/mass spectrometry.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Redox Biol.