Fig. 6

glo2^−/−zebrafish showed a reduction of liver P70–S6 kinase activation while maintaining euglycemia and unchanged AKT phosphorylation.A) The quantification of the pAKT Western blot in glo2^−/− livers showed no change in phosphorylation in the postprandial liver compared to the liver of wildtype zebrafish, indicating intact insulin signaling in glo2^−/− livers. B) The total amount of AKT was unchanged in glo2^−/− and glo2^+/+ zebrafish liver. C) The quantification of the housekeeping gene β-actin showed no significant difference in signal volume, indicating equal loading in all samples. D) The expression of insulin receptor A/B mRNA remained unaltered in glo2^−/− zebrafish. Measured using RT-qPCR, normalized to b2m. N = 4. E)glo2^−/− and glo2^+/+ zebrafish showed similar blood glucose kinetics from fasting to 3 h postprandial state. N = 3-5 adult zebrafish. F)glo2^−/− zebrafish showed a reduction of the phosphorylation of P70–S6 kinase. N = 3/4. G) The relative amount of myo-inositol was increased in glo2^−/− zebrafish. Determined through GC/MS. N = 4. One datapoint represents one zebrafish. N = 3/4. For statistical analysis normality tests were used (A, B, C, D, E, F, G), followed by unpaired t-tests (A, B, C, D, E, F, G). Data presented as mean ± SD. ns = p > 0.05, * = p < 0.05. insr: insulin receptor, pAKT: phosphorylated AKT, tAKT: total AKT, GC-MS: gas chromatography/mass spectrometry.