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Fig. 2

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ZDB-FIG-230608-23
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Jin et al., 2022 - Cdx1b protects intestinal cell fate by repressing signaling networks for liver specification
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Fig. 2

Fig. 2. Cdx1b depletion leads to ectopic expression of a cohort of liver-specific genes in the intestine. AC: Heat maps showing the representative 20 downregulated intestine-specific genes (A), 20 unchanged intestine-specific genes (B), and 20 upregulated liver-specific genes (C) in four cdx1bzju1/zju1 samples (cdx1b) identified by RNA-seq. RNA-seq data for the three WT samples were adopted from our recently published report (Gao et al., 2022). D and E: WISH showing the upregulation of four liver-specific genes (fabp10a, serpina1l, CR626907.1, and apom) (D) and down-regulation of four intestine-specific genes (ldha, chia.1, slc15a1a, and apoea) (E) in the cdx1bzju1/zju1 intestine compared with WT at 5 dpf. F: Representative 3-D images of the DsRed fluorescence in WT and cdx1bzju1/zju1 in the Tg(fabp10a:DsRed) background at 5 dpf. The ectopic expression of RFP in the cdx1bzju1/zju1 intestine is outlined by a dashed yellow line. G: Immunostaining showing the ectopic expression of Fabp10a in the cdx1bzju1/zju1 intestine compared with WT. The Fabp10a-positive signals appeared to be limited in the posterior region of the bulb and anterior region of the mid-intestine. The intestine is outlined with a white dashed line. Red frame, enlarged view on the right. Scale bar, 100 μm. lv, liver; in, intestine.

Expression Data

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Antibody Labeling
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Reprinted from Journal of genetics and genomics = Yi chuan xue bao, 49(12), Jin, Q., Gao, Y., Shuai, S., Chen, Y., Wang, K., Chen, J., Peng, J., Gao, C., Cdx1b protects intestinal cell fate by repressing signaling networks for liver specification, 1101-1113, Copyright (2022) with permission from Elsevier. Full text @ J. Genet. Genomics