Fig. 2

Fig. 2

Fig. 2. Cdx1b depletion leads to ectopic expression of a cohort of liver-specific genes in the intestine. A–C: Heat maps showing the representative 20 downregulated intestine-specific genes (A), 20 unchanged intestine-specific genes (B), and 20 upregulated liver-specific genes (C) in four cdx1b^zju1/zju1 samples (cdx1b) identified by RNA-seq. RNA-seq data for the three WT samples were adopted from our recently published report (Gao et al., 2022). D and E: WISH showing the upregulation of four liver-specific genes (fabp10a, serpina1l, CR626907.1, and apom) (D) and down-regulation of four intestine-specific genes (ldha, chia.1, slc15a1a, and apoea) (E) in the cdx1b^zju1/zju1 intestine compared with WT at 5 dpf. F: Representative 3-D images of the DsRed fluorescence in WT and cdx1b^zju1/zju1 in the Tg(fabp10a:DsRed) background at 5 dpf. The ectopic expression of RFP in the cdx1b^zju1/zju1 intestine is outlined by a dashed yellow line. G: Immunostaining showing the ectopic expression of Fabp10a in the cdx1b^zju1/zju1 intestine compared with WT. The Fabp10a-positive signals appeared to be limited in the posterior region of the bulb and anterior region of the mid-intestine. The intestine is outlined with a white dashed line. Red frame, enlarged view on the right. Scale bar, 100 μm. lv, liver; in, intestine.