Fig. 3

Fig. 3. Depletion of Cdx1b leads to ectopic hepatic gene expression in three major types of gut epithelia cells. A: Visualization of integrated unsupervised clustering of cells in UMAP plot of the sequenced 1229 cells from the WT intestines (Gao et al., 2022) (left UMAP panel) and 1548 cells from cdx1b^zju1/zju1 (mu) intestines (right UMAP panel) at 5 dpf. Annotation of the 15 cell types is shown on the right. B: Feature plot showing the high expression of bhmt and tfa in the group of hepatocyte-like cells in cdx1b^zju1/zju1 (mu) which was not present in WT. C: Colored pie-chart showing the proportion of bhmt⁺/tfa⁺ hepatocyte-like cells (total 633 cells) co-expressing marker genes of enterocytes (fabp2⁺/chia.2⁺), enteroendocrine cells (gcga⁺/insl5a⁺) and goblet cells (agr2⁺) and others. Corresponding number of cells are provided in the dark grey box. D: Histogram showing the percentage of bhmt⁺/tfa⁺ in the group of fabp2⁺/chia.2⁺, gcga⁺/insl5a⁺ and agr2⁺ cells in WT and cdx1b^zju1 (mu), respectively. Note that no bhmt⁺/tfa⁺ cell was detected in WT. E: Whole-mount immunostaining showing that only a proportion of the ectopically expressed RFP (red) was co-expressed with 2F11 (green) in the cdx1b^zju1 intestine which was not observed in WT in the Tg(fabp10a:DsRed) background at 5 dpf. 2F11 staining marks the goblet cells, enteroendocrine cells, biliary cells, and swimming bladder. DAPI stains the nuclei. Scale bar, 100 μm.