FIGURE

Fig. 4

ID

ZDB-FIG-230607-20

Publication

Wada et al., 2022 - Jagged 2b induces intercellular signaling within somites to establish hematopoietic stem cell fate in zebrafish

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Fig. 4

jag2bsgRNA embryos showed reduced wnt16 expression in the somites. (A,B) Expression of desma (a somite marker) and nkx3-1 (a sclerotome marker) in wild-type and jag2bsgRNA embryos. (C) Expression of wnt16 in wild-type and jag2bsgRNA embryos. Graph shows the distribution of embryos exhibiting ‘high’, ‘middle’ or ‘low’ runx1 expression phenotypes. ***P<0.001. (D,E) Expression of dlc and dld in wild-type and jag2bsgRNA embryos. Black arrowheads denote dlc or dld expression in the somite. (F-J) Time course of wnt16 expression in wild-type embryos. (K,L) Expression of wnt16 or both wnt16 and Tp1:GFP in the somites at 15 hpf. A high-magnification view of the boxed region in L is shown to the right. Black and yellow arrowheads indicate wnt16- and Tp1:GFP-expressing cells, respectively. Scale bars: 200 μm (A-J); 10 μm (K,L).

Expression Data

Genes:	desma dlc dld EGFP nkx3-1 wnt16
Fish:	AB AB + CRISPR3-jag2b + CRISPR4-jag2b + CRISPR5-jag2b + CRISPR6-jag2b um14Tg
Knockdown Reagents:	CRISPR3-jag2b CRISPR4-jag2b CRISPR5-jag2b CRISPR6-jag2b
Anatomical Terms:	sclerotome somite
Stage Range:	5-9 somites to 14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + CRISPR3-jag2b + CRISPR4-jag2b + CRISPR5-jag2b + CRISPR6-jag2b
Knockdown Reagents:	CRISPR3-jag2b CRISPR4-jag2b CRISPR5-jag2b CRISPR6-jag2b
Observed In:	somite
Stage:	10-13 somites

Phenotype Detail

Acknowledgments

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