Figure 4
- ID
- ZDB-FIG-230522-42
- Publication
- Almeida et al., 2023 - Early myelination involves the dynamic and repetitive ensheathment of axons which resolves through a low and consistent stabilization rate
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(A) Oligodendrocyte ensheathment dynamics imaging paradigm. (B–E) Lateral images of oligodendrocyte ensheathment dynamics in the spinal cord of living larvae labeled with sox10:eGFP-CAAX and time-lapsed for 15 hours with a 5-minute imaging interval from 2.5-3dpf. (B) Images show the progression of immature sheath accumulation from the first ensheathment attempt in the first panel (set at T0’) to the final ensheathment at T500’ in the bottom panel. Outlined region in first panel is analyzed further in Figure D and E. White arrows identify all immature ensheathments in each frame. (Scale bar = 5 μm). (C) Images showing sheath loss during the stabilization phase. T500’ image is presented again in the top panel and is relabeled to identify future stabilized (cyan) or destabilized (red) ensheathments. This oligodendrocyte has 22 immature sheaths at T500’, 11 of which disappeared, and it has 11 stabilized sheaths at 4dpf in the final panel (Figure 4—figure supplement 2). (Scale bar = 5 μm). (D) Images of the outlined region in the first panel of B visualizing frequent sheath initiation and loss. Each ensheathment is represented with the numbers 1-19 to signify the order that each one appears. The numbers disappear when an ensheathment is lost. Only 5 ensheathment attempts were stabilized out of a total of 19. (Scale bar = 2 μm) (E) Further enlarged images of the outlined region in Figure D (T50’). White arrows point out 3 ensheathment attempts before the final one was stabilized. The YZ images are cross sections of each ensheathment (volume projections in Imaris). A box is drawn around each cylindrical ensheathment in the YZ images. (Scale bar = 2 μm). (See associated source data and supplementary video files).
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