Figure 3
- ID
- ZDB-FIG-230522-38
- Publication
- Almeida et al., 2023 - Early myelination involves the dynamic and repetitive ensheathment of axons which resolves through a low and consistent stabilization rate
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(A) Axonal ensheathment dynamics experimental paradigm. (B–C) Representative lateral images of repetitive ensheathments forming in the spinal cord of living Tg(nkx2.2:eGFP-CAAX) (myelin in green) larvae time-lapsed for 18 hr with a 5 min imaging interval starting at 2.5 days post fertilization (dpf). Axons were labeled with neuroD:tagRFP-CAAX (magenta). Scale bars = 2 µm. (B) Example from larva that was anesthetized in tricaine. (C) Example of larva that was anesthetized in pancuronium bromide. (D) Plot showing the number of times each axonal domain was ensheathed for the tricaine and pancuronium bromide groups (n=37 axonal domains for tricaine, n=29 axonal domains for pancuronium bromide). (E) The same data as in (D), but normalized by axon. When more than one domain was observed on an axon, the total number of ensheathments attempts for each domain was averaged (n=21 axons for tricaine collected from 15 larvae, n=20 axons for pancuronium bromide collected from 16 larvae). For (D) and (E), the dotted lines represent the average number of ensheathment attempts for each group. The error bars represent standard deviation. (F) Pie chart of the percent of axonal domains that were stabilized vs. destabilized. The top pie chart is for the tricaine group and the bottom pie chart is for the pancuronium bromide group. Significance was determined using non-parametric Mann-Whitney tests for (D, E). Significance was determined using a Fisher’s exact test for (F) (see associated source data and supplementary video files).
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