Fig. 4

TDP43^G348C increased sensitivity to ER stress. (A) Transcript levels were evaluated from control or TDP43^G348C larvae, treated or not with the ER stress inducer, tunicamycin, for 24 h. Data from n = 5 were presented as mean ± SEM. Statistical analysis was performed by using ANOVA followed by Tukey's test (*p < 0.05, **p < 0.01 and ***p < 0.001 versus control condition; Фp<0.05, ФФp<0.01). (B) As in (A) for UPR gene targets: BiP and CHOP (**p < 0.01 and ***p < 0.001 versus control condition; ФФp<0.01). (C) As in (A) for NRF2 isoforms: NRF2a and NRF2b (*p < 0.05 and ***p < 0.001 versus control condition). (D) Western blot showing BiP and ATP5α protein levels from control or TDP43^G348C larvae treated or not with tunicamycin for 24 h. (E) Densitometric analysis of BiP protein levels in zebrafish larvae from (D). ATP5α was used as a reference to normalize BiP levels. Data from 5 independent samples were averaged and presented as mean ± SEM. Statistical analysis was performed using a Student's t-test (*p < 0.05).