Fig. 2

PRE-084 ameliorated TDP43^G348C-larvae mobility and axonal projection. (A) Mobility of control larvae, larvae expressing TDP43^G348C alone or treated with 5 μM of PRE-084 during the light/dark phases in the VMR test. The mobility was measured during 1 min each. AU: arbitrary unit. (B) Quantitative analyses from (A) showing the relative mobility of controls, larvae expressing TDP43^G348C alone or treated with PRE-084. Data were the mean of the mobility during the two OFF periods minus ON periods (OFF1-ON1; OFF2-ON2). Relative mobility from >100 larvae was expressed as percent of control and presented as mean ± SEM. Statistical analysis was performed using ANOVA followed by Tukey's test (**p < 0.01; ***p < 0.001). (C) Representative photomicrographs showing three axonal projections in the zone of analysis. Scale bar: 50 μm. (D) Quantitative analyses of axonal projections (as shown in (C)) in control larvae or larvae expressing TDP43^G348C alone or treated with PRE-084. Data from 42 to 54 motor neurons were averaged and presented as mean ± SEM. Statistical analysis was performed using ANOVA followed by Tukey's test (*p < 0.05; ***p < 0.001).