Fig. 5
- ID
- ZDB-FIG-230505-9
- Publication
- Vicente et al., 2022 - Early calcium and cardiac contraction defects in a model of phospholamban R9C mutation in zebrafish
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Cardiac Ca2+ handling and contractility in sibling, Tg-plnbwt and Tg-plnbR9C 9 dpf zebrafish larvae expressing mCyRFP1-GCaMP6f. A) Ratiometric images from beating hearts and their corresponding ventricular Ca2+ traces from representative sibling, Tg-plnbwt and Tg-plnbR9C larvae. B) HR, ventricular CaT amplitude, ventricular Ca2+ rise and decay rates, contraction and relaxation velocities, ESV, EDV, SV, FS, FAC and CO in sibling (n = 26), Tg-plnbwt (n = 17) and Tg-plnbR9C zebrafish larvae (n = 31). Statistical analysis was performed using a one-way ANOVA test with Tukey's multiple comparisons post-test. C) Diagrams of the ventricular diameter (?m) vs. ventricular Ca2+ levels (ratio) from the 3 and 9 dpf representative sibling, Tg-plnbwt and Tg-plnbR9C larvae shown in A. D) Pearson's correlation test between the decay time of CaT and EDV from 9 dpf sibling (n = 26) and Tg-plnbR9C (n = 31) zebrafish larvae. E) Relative expression levels (qPCR) of plnb and atp2a2a in 9 dpf Tg-plnbwt, Tg-plnbR9C and sibling larvae (n = 3, 3 and 4 pools of ten hearts each, respectively). GADPH was used as a normalization control. Data are shown as mean ± SD, (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). |
Reprinted from Journal of Molecular and Cellular Cardiology, 173, Vicente, M., Salgado-Almario, J., Valiente-Gabioud, A.A., Collins, M.M., Vincent, P., Domingo, B., Llopis, J., Early calcium and cardiac contraction defects in a model of phospholamban R9C mutation in zebrafish, 127-140, Copyright (2022) with permission from Elsevier. Full text @ J. Mol. Cell. Cardiol.