FIGURE

Fig. 5

ID

ZDB-FIG-230328-11

Publication

Zhou et al., 2022 - A PI3Kγ signal regulates macrophage recruitment to injured tissue for regenerative cell survival

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Fig. 5

PI3Kγ function is required during the early stages of fin fold regeneration. (a) The experimental scheme of stage‐specific treatment of AS. Group 1 larvae were exposed to AS 0–6 hpa to block macrophage recruitment. Group 2 larvae were exposed to AS 6–12 hpa to allow macrophage recruitment. (b) Macrophage recruitment detected using Tg(mpeg1:mCherry) at 6 and 12 hpa. Macrophages accumulated in the injured site of only group 2 larvae. Scale bar, 50 μm. (c) Quantification of the number of mpeg1 ⁺ cells in the injured site (bracket areas) in (b). (d) Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) analysis of two larval groups at 12 hpa. Scale bar, 50 μm. (e) Quantification of the number of TUNEL‐positive cells in the injured site (bracket areas) in (d). (c and e) Data are presented as the mean ± SEM. Statistical significance was analyzed using one‐way ANOVA. n.s., not significant

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gl23Tg/gl23Tg TU
Condition:	amputation: caudal fin, chemical treatment by environment: (5Z)-5-(quinoxalin-6-ylmethylidene)-1,3-thiazolidine-2,4-dione
Observed In:	regeneration epithelium apoptotic process regeneration epithelium macrophage migration
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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