FIGURE

Fig. 3

ID

ZDB-FIG-230328-9

Publication

Zhou et al., 2022 - A PI3Kγ signal regulates macrophage recruitment to injured tissue for regenerative cell survival

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Fig. 3

Pik3cg knockdown recapitulates the phenotypes in AS‐treated larvae. (a) RT‐PCR analysis of pik3cg mRNA expression in larvae injected with standard control (std) morpholino (MO) or splice site MO. Blocking mRNA splicing causes a premature stop codon and induces mRNA degradation. Arrowhead indicates a minor 750‐bp PCR product that may be derived from unspliced mRNA. (b) Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) analysis of larvae injected with std‐, ATG‐, or splice MOs at 12 hpa. Scale bar, 50 μm. (c) Quantification of TUNEL‐positive cells in the injured site (bracket region) in (b). Statistical significance was tested by one‐way ANOVA; **p < 0.05, ***p < 0.001

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU + MO1-pik3cg TU + MO3-pik3cg
Condition:	amputation: caudal fin
Knockdown Reagents:	MO1-pik3cg MO3-pik3cg
Observed In:	regeneration epithelium apoptotic process
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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