ZFIN Image: Zhou et al., 2022, Fig. 5

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Fig. 5

PI3Kγ function is required during the early stages of fin fold regeneration. (a) The experimental scheme of stage‐specific treatment of AS. Group 1 larvae were exposed to AS 0–6 hpa to block macrophage recruitment. Group 2 larvae were exposed to AS 6–12 hpa to allow macrophage recruitment. (b) Macrophage recruitment detected using Tg(mpeg1:mCherry) at 6 and 12 hpa. Macrophages accumulated in the injured site of only group 2 larvae. Scale bar, 50 μm. (c) Quantification of the number of mpeg1 ⁺ cells in the injured site (bracket areas) in (b). (d) Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) analysis of two larval groups at 12 hpa. Scale bar, 50 μm. (e) Quantification of the number of TUNEL‐positive cells in the injured site (bracket areas) in (d). (c and e) Data are presented as the mean ± SEM. Statistical significance was analyzed using one‐way ANOVA. n.s., not significant

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Acknowledgments

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