FIGURE

Fig. 2

ID

ZDB-FIG-230317-5

Publication

Smith et al., 2021 - Lysosomes and the pathogenesis of merosin-deficient congenital muscular dystrophy

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Lysosome distribution in caf mutants. (A–B) Lamp1-RFP expression in WT embryos shows that lysosomes are distributed throughout the cytoplasm in myofibers but not at myosepta (white arrows) at 3 and 5 dpf. (C–F) Lysosomes redistribute partially to the myosepta (white arrows) in caf mutants at 3 dpf (n = 10 fibers) and 5 dpf (n = 25 fibers), in both attached and detached fibers. Yellow arrows indicate where fibers have detached from the myosepta. (G–H) Lamp1-RFP fluorescence intensity at the myosepta was significantly increased in caf mutants at 3 dpf (*, P = 0.035) and 5 dpf (****, P < 0.0001). Bars represent mean ± SEM.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lama2^{teg15a/teg15a}
Observed In:	lysosome localization myoseptum myotome lysosome
Stage:	Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Hum. Mol. Genet.