FIGURE

Fig. 7

ID

ZDB-FIG-230224-21

Publication

Sahin et al., 2022 - Phenolic Lipids Derived from Cashew Nut Shell Liquid to Treat Metabolic Diseases

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Fig. 7

Figure 7. In vivo testing of CNSL derivatives using transgenic zebrafish that express human PPAR?, PPAR?, or PPAR? reveal tissue-specific activation. Activation of human PPAR in the zebrafish embryo results in GFP expression. Basal activity of PPAR?, PPAR?, and PPAR? is observed with vehicle (DMSO) treatment and is strongly increased in the presence of the full agonist for each receptor. Positive controls are WY (WY14643) for PPAR?, Rosi (rosiglitazone) for PPAR?, and GW (GW0742) for PPAR?, respectively. Compounds were screened at their respective EC50?s determined from their dose?response curves in HEK293 cells with a few exceptions: 4 was screened at 1.5 ?M for all receptors because of toxicity at higher concentrations; for PPAR?, 20 and 27 were screened below their EC50?s due to toxicity at higher concentrations. Each image depicts a representative embryo. Note that embryos incubated with 27 were imaged using a different microscope.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Med. Chem.