FIGURE

Figure 4

ID

ZDB-FIG-230217-13

Publication

Fung et al., 2023 - Expression of the Z Variant of α1-Antitrypsin Suppresses Hepatic Cholesterol Biosynthesis in Transgenic Zebrafish

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Figure 4

Cholesterol biosynthesis is suppressed in ZAAT-expressing zebrafish liver. (A) Mapping of DEGs onto KEGG pathways suggests downregulation of cholesterol biosynthesis (modified from pathways hsa00900 and hsa00100). (B) Total cholesterol was measured in adult zebrafish livers. A total of 18 fish from each genotype, pooled from 5 independent experiments, were used for analysis. Every single point represents the average of two technical replicate measurements for each animal. One-way ANOVA was used to determine statistical significance. Error bars indicate mean ± s.e.m. * p < 0.05, ** p < 0.01. (C) Validation of RNA-seq by qPCR. Expression profiles of six DEGs from RNA-seq and qPCR data were compared (left panel). qPCR expression values were normalised to the housekeeping genes rpl13a and lsm12b. Correlation analysis of RNA-seq and qPCR (right panel), Spearman’s rho, r_s = 0.9429, p = 0.00167.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.