FIGURE

Fig. 3

ID

ZDB-FIG-230212-3

Publication

Rashid et al., 2021 - HI-NESS: a family of genetically encoded DNA labels based on a bacterial nucleoid-associated protein

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

The overlap between H2A-mTurquoise2 (green) and SiR-Hoechst (magenta) signals in HeLa cells (Confocal microscopy, single Z-plane). Nuclei 1 and 2: Extensive nucleolar accumulation of H2A-mTurquoise2 (H2A-mTq2) drowns its fluorescent signal over the rest of the nucleus. Line scans (marked in red with start and end positions indicated with S and E, respectively) across such nuclei show that the H2A-mTq2 signal only recapitulates that of SiR-Hoechst when the line scan does not cross a nucleolus. Nuclei 3, 4, and 5: in cells expressing low levels of H2A-mTq2 (nuclei 3 and 4), and in nuclei with no visible nucleoli (nucleus 5) the distribution of the SiR-Hoechst and H2A-mTq2 signals are comparable.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.