FIGURE

Fig. 4

ID

ZDB-FIG-230212-4

Publication

Rashid et al., 2021 - HI-NESS: a family of genetically encoded DNA labels based on a bacterial nucleoid-associated protein

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Fig. 4

The distribution of mEos3.2-tagged HI-NESS (green) and H2A-mTq2 (magenta) in the nuclei of HeLa cells in culture (Spinning disk microscopy, single Z-plane). In HeLa cells co-expressing mEos3.2-tagged HI-NESS and H2A-mTq2, the fluorescently-labelled histone exhibits extensive nucleolar retention, and consequently, stains the chromosomes with a low signal-to-noise ratio. The decreased nucleolar accumulation of HI-NESS allows chromosomes to be visualised with a higher signal-to-noise ratio. Line scans (marked in red with start and end positions indicated with S and E, respectively) across these nuclei highlight the effect of nucleolar retention on the signal over the rest of the nucleus.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.