Fig. 7

Developmental markers. A and B, Ventral views of whole-mount 6 dpf larval brains, anterior to the top, processed for HuC immunostaining. Vmat2^−/− larval brain displays a similar HuC expression pattern relative to vmat2^+/+ larval brain. Scale bar = 75 µm. C and D, Ventral views of whole-mount 8 dpf larval brains, anterior to the top, processed for notch1a RNA in situ hybridisation (ISH). A weaker signal is seen in the brain of vmat2^−/− larva relative to vmat2^+/+ larval brain. Scale bar = 75 µm. E and F, Dorsal views of whole-mount 6 dpf larval brains, anterior to the top, processed for pax2a RNA in situ hybridisation (ISH). Vmat2^−/− larval brain displays a clearly weaker signal in the midbrain and hindbrain relative to vmat2^+/+ larval brain. Scale bar = 75 µm. G-J, Bar charts showing results of reverse transcription-quantitative PCR (RT-qPCR) assays on larvae of the indicated genotype at 6 dpf. A significant decrease in the expression of notch1a and upregulation of manf transcripts were detected in vmat2^−/− larvae when compared to vmat2^+/+ siblings. No differences between genotypes were detected when pcna and gfap were measured. n = 5 for each genotype. Data are mean ± SEM. Student's t test was used for statistical analysis. *P < .05