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Fig. 1

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ZDB-FIG-230115-49

Publication

Begik et al., 2022 - Nano3P-seq: transcriptome-wide analysis of gene expression and tail dynamics using end-capture nanopore cDNA sequencing

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Fig. 1

Nano3P-seq captures polyadenylated and non-polyadenylated RNAs, while retaining polyA tail length information.

a, Schematic overview comparing three different library preparation methods for studying the transcriptome using nanopore sequencing. RMX, RNA adapter mix (provided with the SQK-RNA002 dRNA-seq library preparation kit); AMX, adapter mix (provided with the SQK-DCS109 dcDNA-seq library preparation kit). b, Nano3P-seq captures a wide range of RNA biotypes in a mouse brain nuclear/mitochondrial RNA sample. c, Integrative Genome Viewer (IGV) snapshot of reads generated with Nano3P-seq, mapped to the Ubb gene, illustrating the diversity of polyA tail lengths captured across different reads. The polyA tail region is shown in green. kbp, kilobase pairs; scaRNA, small Cajal body-specific RNA. d, Scatter plot of log transformed concentrations (amol µl⁻¹) and read counts of sequin genes (Pearson’s R = 0.93, slope = 0.93). Each dot represents a sequin. See also Extended Data Fig. 1h,i.

Source data

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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